Composite

Part:BBa_K1658005:Design

Designed by: Şeniz Yüksel   Group: iGEM15_METU_HS_Ankara   (2015-09-18)


Limonane + GFP regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2358
    Illegal SapI.rc site found at 116


Design Notes

. RBS+Limonene Synthase ,tranitional unit, was ligated with the Reporter biobrick includes RBS(B0032), GFP coding region(E0040) and double terminator(B0010-B0012). The aim was to obtain flourescence under spectrophotometry to measure the production of limonene synthase after ligating with constitutive promoter.

Source

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References